Journal: Scientific reports

Article Title: Differential responses to thrombospondin-1 and PDGF-BB in smooth muscle cells from atherosclerotic coronary arteries and internal thoracic arteries.

doi: 10.1038/s41598-024-66860-x

Figure Lengend Snippet: Figure 1. Morphology and SMC markers in human ITA SMC and ACA SMC. Human SMC derived from 3 distinct ACA donors and 3 distinct ITA donors were plated in T75 flasks with growth medium. Schwann cells and HeLa cells were used as controls. Representative photomicrographs of ACA SMC (A) and ITA SMC (B) taken at subconfluence are shown. Expression of α-actin (C), myh-11 (D), calponin (E), Sox-10 (F) and S100B (G) were determined as described in Methods and a representative blot from 3 independent experiments for each protein is shown. Expression of the senescence biomarkers p16INK4A and p21Cip1 were determined by Western blotting as described in Methods for growth arrested ACA SMC and ITA SMC. Expression was quantified and normalized to β-actin expression. Results from 3 independent experiments are shown in H.

Article Snippet: Recombinant monoclonal anti-transferrin antibody was obtained from Abcam (Boston, MA), a rabbit monoclonal anti-myosin heavy chain antibody was obtained from Invitrogen (catalog number 702544; Waltham, MA) and rabbit monoclonal anti-Sox-10 antibody (catalog number 89356) and rabbit monoclonal anti-S100B antibody (catalog number 9550) were obtained from Cell Signaling Technology (Danvers, MA).

Techniques: Derivative Assay, Expressing, Western Blot

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Figure Lengend Snippet: Effect of SME on the retinal inflammation of STZ rats.

Article Snippet: The following primary antibodies were used: glial fibrillary acidic protein (anti-GFAP; MAB360; Chemicon International, Inc., Temecula, CA, USA); vascular endothelial grown factor (anti-VEGF; sc-7269; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), S100 calcium-binding protein B (anti-S100B; ab52642; Abcam PLC, Cambridge, UK), and nuclear factor kappa-light-chain-enhancer of activated B cells (anti-NF-κB p65; sc-8008).

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Figure Lengend Snippet: The S100 calcium-binding protein B (S100B) levels in the retina of the STZ. Retina sections were stained with anti-S100B in the NG ( A ), STZ ( B ), STZ-AG ( C ), and STZ-SME ( D ) groups. ( E ) Shows the box-and-whisker plot of the IOD for S100B protein staining in the three retinal layers for all groups. * p < 0.01 compared to NG; α p < 0.01 compared to STZ; and β p < 0.01 compared to STZ-AG. Magnification 200×.

Article Snippet: The following primary antibodies were used: glial fibrillary acidic protein (anti-GFAP; MAB360; Chemicon International, Inc., Temecula, CA, USA); vascular endothelial grown factor (anti-VEGF; sc-7269; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), S100 calcium-binding protein B (anti-S100B; ab52642; Abcam PLC, Cambridge, UK), and nuclear factor kappa-light-chain-enhancer of activated B cells (anti-NF-κB p65; sc-8008).

Techniques: Binding Assay, Staining, Whisker Assay

Journal: Cell Reports Methods

Article Title: A fluorescent splice-switching mouse model enables high-throughput, sensitive quantification of antisense oligonucleotide delivery and activity

doi: 10.1016/j.crmeth.2023.100673

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Article Snippet: Rabbit anti-S100B , Proteintech , 15146-1-AP; RRID:AB_2254244.

Techniques: Recombinant, Electron Microscopy, Ligation, Sequencing, Expressing, Software, Imaging